The Serum Triglyceride Quantification Kit is a simple, colorimetric assay that quantitatively measures the amount of triglyceride in plasma, serum, and lysates in a 96-well microtiter plate format. The kit measures triglyceride concentration in serum, plasma, and lysates by a coupled enzymatic reaction system. First, lipase hydrolyzes the triglyceride ester bond, yielding glycerol. The glycerol is then phosphorylated and oxidized, producing hydrogen peroxide which reacts with the kit’s Colorimetric Probe (absorbance maxima of 570 nm). Each kit provides sufficient reagents to perform up to 100 assays, including blanks, triglyceride standards, free glycerol controls and unknown samples. The kit contains a triglyceride standard and has a detection sensitivity limit of ~10 µM (1 mg/dL).